FOOD Barley 6,000 yrs Old and evidince of cultivated plants 23,000 yrs ago found in Israel

Melodi

Disaster Cat
While I thought the 6 thousand year old barley to be amazing enough, the mention of a find last year officially pushing back plant domestication in the Middle East to 23,000 years ago stunning.

This also means the great stone monuments found in Turkey that are at least 12 to 14,000 years old, could easily have been built by people who understood agriculture, perhaps not a grand scale but at least growing gardens and creating enough surplus to support skilled stone masons and other workers part of the year.]


http://www.timesofisrael.com/6-millennia-old-but-almost-fresh-masada-seeds-unravel-barleys-origins/
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Israel & the Region
6 millennia old but ‘almost fresh,’ Masada seeds unravel barley’s origins
Grains found in Judean Desert, the oldest plant DNA ever sequenced, point to cereal’s domestication in Jordan Valley, scientists say
By Ilan Ben Zion July 18, 2016, 10:55 pm

A new study has allowed scientists to peer thousands of years back in time via a grain of barley found in the Judean Desert.

Barley seeds, dated to 6,000 years ago, have become the oldest plant genome to be sequenced, an international team of researchers announced in a journal article published Monday. Analysis of the 6,000-year-old cereals supports the hypothesis that the key crop was domesticated thousands of years ago in the Jordan Valley.

A team of scientists from Israel, Germany, the United Kingdom and the US employed a wide array of disciplines — archaeology, archaeobotany and genetics — to study the material found in the Yoram Cave. The findings were released in the academic journal Nature Genetics.

The Chalcolithic kernels were discovered in a cavern overlooking the Dead Sea on the southern end of Masada, a mountaintop better known for Jewish rebels’ last stand against the Roman Empire in the first century CE.

The arid climate and precipitous cliff left the grains preserved for millennia. Ehud Weiss of Bar-Ilan University, one of the heads of the study, told The Times of Israel that whereas most ancient kernels are found charred and useless for DNA study, those excavated from the cave on Masada by a Hebrew University team “looked almost alive, almost fresh.”

Their immaculate preservation allowed scientists to “read the DNA from these seeds” and determine that they were domesticated locally, he said.

Weiss said that the barley found at Masada could only have been grown at least 20 kilometers (12 miles) from the remote mesa. Hebrew University archaeologist Uri Davidovitch posited that the people who brought it to the cave may have fled some unknown catastrophe and sought refuge in the desert, just like the mountaintop’s Jewish inhabitants thousands of years later.

Radiocarbon dating determined the seeds were 6,000 years old, grown several millennia after humans residing in the Fertile Crescent first domesticated grains such as barley and wheat around 10,000 years ago.

Until now, corn was the only ancient grain whose genetic fingerprint was fully mapped out. Barley’s genome was only studied through modern samples.

The seeds found in at Masada are “much closer to the time and place of domestication,” Weiss said. They are a “time capsule” that gives scientists a shortcut around 6,000 years of genetic mutation and offers insight into what the ancients ate.

Sequencing prehistoric barley is “just the beginning of a new and exciting line of research,” Verena Schuenemann of Tubingen University, one of the heads of the study, said.

Examination of the barley grains’ genome found they are significantly different from wild varieties, but similar to modern cultivars still grown in the region. The finding bolsters the hypothesis that barley was domesticated in the Jordan Valley, researchers said.

Last year a study by researchers from Tel Aviv University, Harvard University and Bar Ilan found cultivated plants in the Galilee dating back 23,000 years, pushing back the origins of domesticated crops at least 11,000 years.

A study published in 2015 said genetic analysis of barley varieties pointed to domestication of the grain occurring at several points across the Mideast in prehistory.

“DNA-analysis of archaeological remains of prehistoric plants will provide us with novel insights into the origin, domestication and spread of crop plants,” Schuenemann said in a statement.
 

Melodi

Disaster Cat
Any hops & yeast found onsite?
Way too early for hops and yeast was made in the process of making early barley beer; which we studied when we were researching the novel about Gilgamesh my husband wrote; these people would probably have used the same basic method of making special "bread" loaves; firing them a certain way and then letting them make a sort of sour mash to generate wild yeast - there were other steps I don't remember but the whole process is on-line and there have been craft beer hobby folks (and I think at least on micro-brewery) that have tried to recreate it.

One interesting thing that isn't mentioned in this article (probably because what was found was just a forgotten storage bin, not a complete settlement) is that in Mesopotamia at least; the greatest portion of early grain production seems to have gone into making beer/ale and fruit beer (brewing it all together) rather than bread.

There are even hints in the record that in some places; the cultivation of grains was more about making booze than feeding people bread and porridge although since a recent article (which I think I posted here about a year ago) showed people eating porridge 28,000 years ago in Europe (probably before domestication) those foods also go way back.
 

tanstaafl

Has No Life - Lives on TB
I thought the climate in the Middle East was much wetter at one time than now, and that wetter climate has been widely accepted as why agriculture and domestication of animals took off so well in the Fertile Crescent and its environs in the first place. In fact, as far as I know the generally accepted theory is that humans had much to do with turning the Middle East into the arid climate it is today (that is, overgrazing with their new domesticated critters, cutting down the trees, overuse of the water systems, etc.). Claiming the exceptionally dry climate of the area preserved the seeds for tens of thousands of years would seem to contradict climate claims that have been widely accepted.
 

Melodi

Disaster Cat
Some places were wetter, especially Egypt if you go back about 10,000 years; other places not so much, this particular area and some parts of Mesopotamia (area of parts of modern Turkey, Iraq and Iran0 were very dry.

In fact in order to grow sizable crops serious irrigation was needed in the fertile crescent areas; and there is a strong theory that it may be cooperation to bring about irrigation that brings some of the earliest advanced towns and cities (this shows up in Peru and Egypt as well).

This barley was found in a very dry cave and was probably someone's storage bin during a crises that was never used; caves even in wet climates can have very dry (or sometimes cold) micro-climates) that allows for astonishing preservation of usually hard to find items like textiles, grains, leather and sometimes even human or animal remains (usually naturally mummified or frozen; sometimes it is salt that does the preservation).

Parts of Israel and the surrounding areas have areas that have been rather dry and aired for thousands of years; that doesn't mean it never rains or that agriculture is not possible; it just means the air over-all is dry enough to allow some protected areas like caves, ancient dried wells, cliff hangings or even long buried living sites to be better preserved and dried out than the would say in Germany or England.

But even in those two places which really are very wet (and have been mostly since the stone age) you get the occasional pocket of dry air and good luck; though more finds in Northern Europe come from bogs, lakes or even salt mines. Wet preservation depends on the acid levels in the water; sometimes you get plant products (linen, hemp etc) that survives and others animal (wool, leather, human hair etc).

And climates DO change which is very different from the whole media "climate change hype" the Middle East has gone through varying periods of having more or less rainfall; and was probably more temperate when the Great Ice sheets were over Europe - which may have led to agriculture 23,000 years ago being easier (or at least planned gardens) but again sometimes the air in a cave or other long-term storage area might stay dry enough for thousands of years to protect something; especially if the site was either intentionally buried (a traditional way of preserving food) or quickly covered by dust or other material that "sealed" it in.

Finally, technology is rapidly improving as are excavation techniques (as well as the cost of many items needed to do good field work) so while in the past it was common to read "when we opened the cave bits of what appeared to be textiles or strings of leather were seen for a few moments but they rapidly fell to dust when exposed to the fresh air.

That still happens sometimes (Ireland lost a full Viking Tunic with silver decorations a few years ago; it was also in a cave on a wet sea shore but the dry cave air had preserved it - but the young student got excited and didn't call for help; most of the fabric fell apart in a couple of days, though of course the silver decorations remain.

But if that had been a planned and full expedition, all work would have stopped while the site was secured and the items not touched until a professional could get there with a properly sealed container that the entire artifact (often with the ground under and beside it) was dug out and placed in; then returned to a modern lab for the actual excavation.

Ireland has had several major climate changes (from warmer, to little ice age, to warmer again) since the Viking Age, but the air in that cave stayed dry enough to keep a hand woven tunic (inside out so probably pulled quickly over the head of someone running from something) thrown into; intact for about 1,000 years.

Dryer areas like the South Western US or parts of the Near and Middle East; make this happen a lot more often (though it is still rare enough to be thrilling).
 

Faroe

Un-spun
Way too early for hops and yeast was made in the process of making early barley beer; which we studied when we were researching the novel about Gilgamesh my husband wrote; these people would probably have used the same basic method of making special "bread" loaves; firing them a certain way and then letting them make a sort of sour mash to generate wild yeast - there were other steps I don't remember but the whole process is on-line and there have been craft beer hobby folks (and I think at least on micro-brewery) that have tried to recreate it.

One interesting thing that isn't mentioned in this article (probably because what was found was just a forgotten storage bin, not a complete settlement) is that in Mesopotamia at least; the greatest portion of early grain production seems to have gone into making beer/ale and fruit beer (brewing it all together) rather than bread.

There are even hints in the record that in some places; the cultivation of grains was more about making booze than feeding people bread and porridge although since a recent article (which I think I posted here about a year ago) showed people eating porridge 28,000 years ago in Europe (probably before domestication) those foods also go way back.

Melodi, if you haven't come across it yet, Stephen Harrod Buhner's Sacred and Herbal Healing Beers might be of interest to you both. One of my all-time favourite books.
 

tanstaafl

Has No Life - Lives on TB
Melodi, that was a good reply to my comments about a wetter Middle East climate in the past. There are exceptions to every general statement, of course, but I'd still want evidence that the cave in question is in fact an exception in terms of its dryness. Either that or evidence that the area's climate was NOT wetter in the years covering the suggested storage of the seeds. In my book you don't get to have it both ways ... that for some theories the general area was always arid but for other theories it was wet and green. It seems to me that quite a lot of evidence exists for the climate having been much more forgiving in places where they're now finding very ancient ruins.

Sometimes even science professionals too easily fall into the habit of thinking everything has always been like today, when in reality that's not remotely the case. For example, more than once I've read people sneer at claims of ancient widespread sea travel by asking, "Where are all the boats, then?" Apparently not realizing that the coasts (where the boats would naturally be) were once MUCH different from present day coastlines, and who the heck hauls boats hundreds of miles from the coast and away from rivers unless they HAVE to?
 

Melodi

Disaster Cat
Well, I haven't seen the report on that particular cave but the cave here on the coast of Ireland with its ranging winds, gale force storms, sea spray and year round humid gloom; was dry enough in the interior to preserve an intact 1,000 year old woven shirt....

Micro climates matter a lot and caves have some of the best; the amazing textiles from early Iron Age Germany were found deep in a mine shaft (though salt helped with that preservation) I tell my students when I show them pictures of bands woven with costly Chinese silk; I know at least one husband who got a burned dinner when his wife asked him where his best shirt was and he mumbled about it being hot in the mine and she screamed "you wore what, to do what and you left it WHERE?"....burned barley tonight...
 

tanstaafl

Has No Life - Lives on TB
We have microclimates out the wazoo here in the Pacific Northwest. On the Olympic Peninsula we have the rainiest place in the lower 48 and then a few dozen miles away they have to irrigate to grow things (the rain shadow from the Olympic Mountains on the peninsula). It's amazing what a relatively isolated large bump in the surface (i.e., even a small ridge standing alone) or a big hole in the ground (in my case, the west end of the Columbia Gorge just down the road) can do to the flow of local winds and therefore the local weather. There's an interesting book about it titled "Rains All The Time: A Connoisseur's History Of Weather in the Pacific Northwest" (Davide Laskin, 1997).

But I can't recall ever reading anything about how long microclimates can last over hundreds or thousands of years. You push or pull on one part of the balloon and there are impacts to the shape of the rest of the balloon. We know now more about what impact El Nino can have half a world away even on things as traditionally reliable as monsoons, so I'd think things in El Nino's class (global weather changers) must also have an impact on microclimate stability at the local level. Which has a direct bearing on how long a cave like that could retain its dryness over the tens of thousands of years required for the barley seeds to have survived in good enough shape to be able to decode their DNA.

Still, no matter what has changed long-term in the area's climate around the cave, if they did their research right and the seeds are really that old (which is where scientific peer review is supposed to kick in) then it's really just an academic debate about the why. I love it in "The War of the Worlds" when the scientist is completely flummoxed by the existence of the Martians on a supposedly dead planet: "The chances of anything coming from Mars are a million to one ... but still they come!" Reality ALWAYS trounces speculation (or theory)! :)
 

Melodi

Disaster Cat
I agree about micro-climates above ground, although there are a few of those that do manage to last thousands of years; but for the most part they ebb and change a lot.

But caves, and sometimes underground chambers can stay pretty steady for thousands of years; that's why on rare occasions finds of very well preserved seeds or textiles do still happen; this was in a cave and in a storage pit; most of the viable ancient grains have been found in similar places including the tombs of ancient Egypt.

Also, preservation of animal or plant matter can be very strange and a matter of just plain luck; in an echo of Jurassic Part; a few years ago real tissue was found buried in the middle of a T Rex bone; and several other examples I believe have been found since then. They are extremely rare but they do happen, this is the most recent theoretical explanation as to how it can happen..

http://www.livescience.com/41537-t-rex-soft-tissue.html

I'll post the entire article in a separate post here...

And I totally agree that climate changes a lot; it does it constantly, but some areas of the world have had more similar patterns than other for the last 50,000 years or so; some places like parts of Europe, Asia and North America have varied wildly as ice ages came and went; other places like some pockets in the Middle East and Africa (and I gather Indonesia) not as much.

I also love the "its a million to one" opps we got the one!...makes history and social sciences more fun!
 

Melodi

Disaster Cat
Controversial T. Rex Soft Tissue Find Finally Explained
By Stephanie Pappas, Senior Writer | November 26, 2013 07:01pm ET



Controversial T. Rex Soft Tissue Find Finally Explained
Artwork by Scott Hartman reveals the bone structure of T. rex.
Credit: © Scott Hartman / All rights reserved

The controversial discovery of 68-million-year-old soft tissue from the bones of a Tyrannosaurus rex finally has a physical explanation. According to new research, iron in the dinosaur's body preserved the tissue before it could decay.

The research, headed by Mary Schweitzer, a molecular paleontologist at North Carolina State University, explains how proteins — and possibly even DNA — can survive millennia. Schweitzer and her colleagues first raised this question in 2005, when they found the seemingly impossible: soft tissue preserved inside the leg of an adolescent T. rex unearthed in Montana.

"What we found was unusual, because it was still soft and still transparent and still flexible," Schweitzer told LiveScience.

T. rex tissue?

The find was also controversial, because scientists had thought proteins that make up soft tissue should degrade in less than 1 million years in the best of conditions. In most cases, microbes feast on a dead animal's soft tissue, destroying it within weeks. The tissue must be something else, perhaps the product of a later bacterial invasion, critics argued.

Then, in 2007, Schweitzer and her colleagues analyzed the chemistry of the T. rex proteins. They found the proteins really did come from dinosaur soft tissue. The tissue was collagen, they reported in the journal Science, and it shared similarities with bird collagen — which makes sense, as modern birds evolved from theropod dinosaurs such as T. rex.

The researchers also analyzed other fossils for the presence of soft tissue, and found it was present in about half of their samples going back to the Jurassic Period, which lasted from 145.5 million to 199.6 million years ago, Schweitzer said.

"The problem is, for 300 years, we thought, 'Well, the organics are all gone, so why should we look for something that's not going to be there?' and nobody looks," she said.

The obvious question, though, was how soft, pliable tissue could survive for millions of years. In a new study published today (Nov. 26) in the journal Proceedings of the Royal Society B: Biological Sciences, Schweitzer thinks she has the answer: Iron.

Iron lady

Iron is an element present in abundance in the body, particularly in the blood, where it is part of the protein that carries oxygen from the lungs to the tissues. Iron is also highly reactive with other molecules, so the body keeps it locked up tight, bound to molecules that prevent it from wreaking havoc on the tissues.

After death, though, iron is let free from its cage. It forms minuscule iron nanoparticles and also generates free radicals, which are highly reactive molecules thought to be involved in aging.

"The free radicals cause proteins and cell membranes to tie in knots," Schweitzer said. "They basically act like formaldehyde."

Formaldehyde, of course, preserves tissue. It works by linking up, or cross-linking, the amino acids that make up proteins, which makes those proteins more resistant to decay.

Schweitzer and her colleagues found that dinosaur soft tissue is closely associated with iron nanoparticles in both the T. rex and another soft-tissue specimen from Brachylophosaurus canadensis, a type of duck-billed dinosaur. They then tested the iron-as-preservative idea using modern ostrich blood vessels. They soaked one group of blood vessels in iron-rich liquid made of red blood cells and another group in water. The blood vessels left in water turned into a disgusting mess within days. The blood vessels soaked in red blood cells remain recognizable after sitting at room temperature for two years. [Paleo-Art: Illustrations Bring Dinosaurs to Life]

Searching for soft tissue

Dinosaurs' iron-rich blood, combined with a good environment for fossilization, may explain the amazing existence of soft tissue from the Cretaceous (a period that lasted from about 65.5 million to 145.5 million years ago) and even earlier. The specimens Schweitzer works with, including skin, show evidence of excellent preservation. The bones of these various specimens are articulated, not scattered, suggesting they were buried quickly. They're also buried in sandstone, which is porous and may wick away bacteria and reactive enzymes that would otherwise degrade the bone.

Schweitzer is set to search for more dinosaur soft tissue this summer. "I'd like to find a honking big T. rex that's completely articulated that's still in the ground, or something similar," she said. To preserve the chemistry of potential soft tissue, the specimens must not be treated with preservatives or glue, as most fossil bones are, she said. And they need to be tested quickly, as soft tissue could degrade once exposed to modern air and humidity.

Importantly, Schweitzer and her colleagues have figured out how to remove the iron from their samples, which enables them to analyze the original proteins. They've even found chemicals consistent with being DNA, though Schweitzer is quick to note that she hasn't proven they really are DNA. The iron-removing techniques should allow paleontologists to search more effectively for soft tissue, and to test it when they find it.

"Once we can get the chemistry behind some of these soft tissues, there's all sorts of questions we can ask of ancient organisms," Schweitzer said.

Editor's Note: This article was updated at 2pm Eastern Nov. 28 to correct unclear language about proteins and DNA.

Follow Stephanie Pappas on Twitter and Google+. Follow us @livescience, Facebook & Google+. Original article on LiveScience.
http://www.livescience.com/41537-t-rex-soft-tissue.html
 
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